Composite

Part:BBa_K2296006:Design

Designed by: Fion Chu   Group: iGEM17_Cornell   (2017-10-21)


Constitutive Promoter - RBS - roGFP2 - Orp1 C82S


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1343


Design Notes

We put the CDS of roGP2 and Orp1 C82S, with a linker in between the two CDS, under a constitutive promoter and an RBS.


Source

gBlock

References